1866-15-5 S-Acetylthiocholine iodide AKSci J93481
 
 
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  J93481    AKSci Bulk Reagent
S-Acetylthiocholine iodide, 95%
 
(2-Mercaptoethyl)trimethylammonium iodide acetate
IDENTITY
CAS Number:1866-15-5
MDL Number:MFCD00011819
MF:C7H16INOS
MW:289.18
EINECS:217-474-1
BRN:3916578
SPECIFICATIONS & PROPERTIES
Min. Purity Spec:95%
Physical Form (at 20°C):Solid
Melting Point:205-210°C
Long-Term Storage:Store long-term at 2-8°C
DOT/IATA TRANSPORT INFORMATION
UN #:UN2811
Hazard Class:6.1; Poison
Packing Group:III
A hazmat fee may apply, depending on the amount ordered.

REVIEW
 Acetylthiocholine iodide is used as a synthetic substrate for the assay of acetylcholine esterase (E.C. 3.1.1.7), replacing the natural substrate acetylcholine. This enzyme hydrolyses the substrate to yield acetate and thiocholine. The free thiol group of thiocholine reacts with 5,5'-dithiobis-2-nitrobenzoic acid (DTNB, Ellman's reagent, producing the yellow 4-nitrothiolate anion. The release of this yellow anion is followed at 412 nm. The assay may be performed at 30°C in 0.1 M sodium phosphate buffer with 10 mM DTNB and 12.5 mM acetylthiocholine iodide.

REFERENCES
[1]John, R.A. (1993) Photometric assays in Enzyme Assays: A Practical Approach p. 81 (Eisenthal, R. & Danson, M.F. eds.) Oxford University Press; Oxford, New York, Tokyo.
[2] Koitka M, Höchel J, Obst D, Rottmann A, Gieschen H, Borchert HH. Determination of rat serum esterase activities by an HPLC method using S-acetylthiocholine iodide and p-nitrophenyl acetate. Anal Biochem. 2008 Oct 1;381(1):113-22.
[3] Hamers T, Molin KR, Koeman JH, Murk AJ. A small-volume bioassay for quantification of the esterase inhibiting potency of mixtures of organophosphate and carbamate insecticides in rainwater: development and optimization. Toxicol Sci. 2000 Nov;58(1):60-7.

GLOBALLY HARMONIZED SYSTEM (GHS)

Pictograms

Signal Word
Danger

Hazard Statements
H301+H311; H315; H319; H335

Precautionary Statements
P261; P264; P270; P271; P280; P301+P310; P302+P352; P304+P340; P305+P351+P338; P312; P322; P330; P332+P313; P337+P313; P361; P403+P233; P405; P501

Current as of October 1, 2022


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CATEGORIES
 APIs and Bioactives > AChR Agonists


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