A hazmat fee may apply depending on the amount ordered, as indicated in the list of package sizes.
REVIEW Acetylthiocholine iodide is used as a synthetic substrate for the assay of acetylcholine esterase (E.C. 3.1.1.7), replacing the natural substrate acetylcholine. This enzyme hydrolyses the substrate to yield acetate and thiocholine. The free thiol group of thiocholine reacts with 5,5'-dithiobis-2-nitrobenzoic acid (DTNB, Ellman's reagent, producing the yellow 4-nitrothiolate anion. The release of this yellow anion is followed at 412 nm. The assay may be performed at 30°C in 0.1 M sodium phosphate buffer with 10 mM DTNB and 12.5 mM acetylthiocholine iodide.
REFERENCES
[1]
John, R.A. (1993) Photometric assays in Enzyme Assays: A Practical Approach p. 81 (Eisenthal, R. & Danson, M.F. eds.) Oxford University Press; Oxford, New York, Tokyo.
[2]
Koitka M, Höchel J, Obst D, Rottmann A, Gieschen H, Borchert HH. Determination of rat serum esterase activities by an HPLC method using S-acetylthiocholine iodide and p-nitrophenyl acetate. Anal Biochem. 2008 Oct 1;381(1):113-22.
[3]
Hamers T, Molin KR, Koeman JH, Murk AJ. A small-volume bioassay for quantification of the esterase inhibiting potency of mixtures of organophosphate and carbamate insecticides in rainwater: development and optimization. Toxicol Sci. 2000 Nov;58(1):60-7.
These products are explicitly not intended to be used in foods and/or cosmetics and/or drugs (human and veterinary) and/or consumer products and/or biocides and/or pesticides of any kind unless explicitly stated otherwise.
Products are not sold to individuals. We do not ship to residential addresses. Consumer orders will be cancelled without notice.
New customers undergo an internal onboarding process. As part of this process, new customers may be asked for more information. Additional restrictions may apply.
Add to Favorites 
Delete from Favorites 
