119171-73-2 10-Acetyl-3,7-dihydroxyphenoxazine AKSci 3273AA
 
 
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  3273AA    AKSci Reference Standard
10-Acetyl-3,7-dihydroxyphenoxazine
, 98% (HPLC)
 
Ampliflu Red




IDENTITY
CAS Number:119171-73-2
MDL Number:MFCD00467861
MF:C14H11NO4
MW:257.24
SPECIFICATIONS & PROPERTIES
Purity:98% (HPLC)
Spectra:LCMS, HPLC
Physical Form:Light brown powder
Long-Term Storage:Store long-term at 2-8°C

REVIEW

 10-Acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.

REFERENCES
[1]I. Snyrychova et al.; Phys. Plantarum, 135(1), 1-18 (2009)
[2] J.G. Montay et al.; J. Immunol. Meth. 202, 133 (1997)
[3] M. Zhou et al.; Anal. Biochem. 253, 162 (1997)

GHS

Pictograms

Signal Word
Warning

Hazard Statements
H302

Precautionary Statements
P264; P270; P301+P312; P330; P501


Current as of March 19, 2019

AKSci Reference Standards are high-purity, low-impurity compounds suitable for use as standards.


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