494-38-2 Acridine orange base AKSci L916
 
 
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  L916    
Acridine orange base
, 95% (HPLC)
 
N3,N3,N6,N6-tetramethyl-3,6-acridinediamine, C.I. 46005B, NSC 194350




IDENTITY
CAS Number:494-38-2
MDL Number:MFCD00005029
MF:C17H19N3
MW:265.35
SPECIFICATIONS & PROPERTIES
Min. Purity Spec:95% (HPLC)
Physical Form:Brown to red powder
Melting Point:165°C
Boiling Point:468.578°C
Flash Point:237.187°C
Density:1.17
Refractive Index:1.71
Long-Term Storage:Store long-term in a cool, dry place
Note:Emission: 25 nm (dsDNA), 650 nm (ssDNA and RNA), 590 nm (low pH); Excitation: 502 nm (dsDNA), 460 nm (ssDNA and RNA), 475 nm (low pH)
HAZARDOUS MATERIALS INFORMATION
Not hazardous material

BIOLOGICAL INFO
Solubility:DMF: 2 mg/ml, DMSO: 20 mg/ml, Ethanol: 0.3 mg/ml, PBS (pH 7.2): 1 mg/ml

REVIEW

 A fluorescent cationic dye useful for cell cycle determination. Emission: 525 nm (dsDNA), 650 nm (ssDNA and RNA), 590 nm (low pH), Excitation: 502 nm (dsDNA), 460 nm (ssDNA and RNA), 475 nm (low pH).

REFERENCES
[1]Virant-Klun, I., Tomazevic, T., and Meden-Vrtovec, H. Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos. J.Assist.Reprod.Genet. 19(7), 319-328 (2002).
[2] Han, J., and Burgess, K. Fluorescent indicators for intracellular pH. Chemical Reviews 110(5), 2709-2728 (2010).
[3] Thomé, M.P., Filippi-Chiela, E.C., Villodre, E.S., et al. Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy. J. Cell Sci. 129(24), 4622-4632 (2016).

GHS

Pictograms

Signal Word
Warning

Hazard Statements
H315; H319; H335

Precautionary Statements
P261; P264; P271; P280; P302+P352; P304+P340; P305+P351+P338; P312; P321; P332+P313; P337+P313; P362; P403+P233; P405; P501


Current as of July 31, 2021


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